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Researchers conducted the first comprehensive cytogenetic analysis of Betula klokovii, a rare endemic birch species from Ukraine, confirming it is a tetraploid with 56 chromosomes (2n = 4x = 56). Using flow cytometry, they determined the nuclear DNA content ranges from 1.93-1.99 picograms for fresh specimens and 2.15-2.53 picograms for herbarium material. The team also identified one seedling with reduced DNA content (1.46 picograms), likely representing a triploid hybrid between B. klokovii and the common birch B. pendula.
Why it matters
This baseline genomic data is essential for conservation efforts of this rare endemic species and helps clarify the taxonomic relationships within European birches. The identification of natural hybridization with common birch has implications for understanding genetic diversity and developing protection strategies for threatened birch populations.
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⚠️ Preprint – Noch nicht peer-reviewed
Dieser Artikel wurde noch nicht von unabhängigen Experten begutachtet. Die Ergebnisse sind vorläufig und sollten mit Vorsicht interpretiert werden.
This study presents the first report on chromosome number and genome size estimate for rare endemic birch species Betula klokovii Zaverucha from Ukraine. Karyological analysis of metaphases revealed a total of 56 chromosomes, confirming a tetraploid cytotype (2n = 4x = 56). Concurrently, flow cytometry (FCM) was utilized to conduct a precise genome estimation, yielding a nuclear DNA content of 2C = 1.93-1.99 pg for fresh material and 2.15-2.53 pg for herbarium. In addition, we identified one seedling with lower DNA content of 2C = 1.46 pg that most likely corresponds to F1 triploid hybrid between B. klokovii and B. pendula Roth. The results contribute significantly to our understanding of genomic evolution within the genus Betula L. and provide important insights into the taxonomy and conservation genetics of rare European birch taxa.