[ASAP] Nanosensor Quantifying Lysosomal Glycosidase Secretion from Single Living Cells

AI Insight

This study presents a nanosensor designed to detect and quantify the secretion of lysosomal glycosidases from individual living cells. The sensor enables real-time measurement of enzymatic activity at the single-cell level, overcoming the averaging limitations of bulk assays. By targeting lysosomal glycosidases, which are enzymes involved in the breakdown of glycosidic bonds within lysosomes, the nanosensor provides spatially and temporally resolved data on cellular secretion events.

Why it matters

Lysosomal enzyme dysfunction is associated with lysosomal storage disorders and other diseases, so tools capable of monitoring these enzymes at single-cell resolution could advance early diagnostics and our understanding of disease mechanisms. This technology may also support drug screening efforts targeting lysosomal pathways.