Sinefungin is a potent nucleoside antimetabolite of S-adenosylmethionine (SAM), yet its biosynthesis has remained unclear for decades. Here we detail the identification and characterization of the…
-Lipoic acid (LA) is widely included in mitochondrial cocktails recommended to patients with primary mitochondrial disorders, yet its mechanism of action remains unclear. Here, we define the…
CRISPR-Cas9 perturbation screens coupled with single-cell multi-omic profiling enable dissection of gene regulatory mechanisms, yet existing analyses largely quantify total perturbation effects and…
Plant viruses have evolved diverse strategies to facilitate their movement and survival within the host. Among them, geminiviruses co-opt host cellular machinery to replicate and disseminate….
Native mass spectrometry (nMS) is well established for measuring protein masses and stoichiometries using nano-electrospray ionization (nESI), yet salt adduction and source activation energies can…
The nucleolus is widely regarded as a specialized compartment for RNA polymerase I (Pol I)-driven ribosomal RNA transcription and ribosome biogenesis. Yet the presence of ‘atypical nucleoli’, or…
Drug-induced seizures remain a major safety concern in drug development, yet human seizure liability is difficult to predict using conventional preclinical models. Here, we evaluated whether…
Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have enabled atlas-scale cellular cartography, with consortium efforts now assembling millions of cells across diverse tissues,…
Animal tissues have diverse architectures and cell behaviors across the epithelial-mesenchymal spectrum. Cell adhesion mediated by classical cadherins is foundational. Cadherins nucleate complexes of…
More than 40 years after the end of commercial whaling, new research reveals a recent increase in sightings of the world’s two largest whale species in the southeastern Atlantic. The findings,…